Hawaii Pacific University | Shared Instrumentation Facilities
Established in 2005, the Shared Instrumentation Facility (SIF) houses the major scientific instruments at HPU and brings together an essential collection of labs, support equipment, staff, and other research resources. The SIF seeks to maintain high functioning research labs, provide uninterrupted access to and training on the instruments, and promote a safe working environment through extensive training and adherence to best practices.
The SIF supports biomedical and other multidisciplinary research and integrates high tech instrumentation into HPU's science lab courses. The SIF includes instrumentation and laboratory space on the Hawaii Loa campus, including cell biology (AC 102A), chemistry (AC 102D), and multi-purpose (AC 214) research laboratories. The SIF recently added new labs in the Model Progress building in downtown Honolulu, representing a 45% expansion of HPU's biomedical research laboratories. The new space
The overarching goal of the SIF is to provide outstanding instrumentation and laboratory facilities to support chemical, biological, biomedical, and marine science research, and to support extraordinary educational activities for HPU students. Specifically, the SIF seeks to
- Support state-of-the-art instrumentation and research labs at HPU to cultivate innovative and forward-looking research, while creating exceptional student research opportunities
- Integrate the same
high tech scientific instruments into our teaching labs to provide direct hands-on experience to all students in our laboratory courses - Provide training that promotes safe and effective use of the facilities
- Provide our expertise to support and enrich student and faculty research
The SIF represents an excellent example of synergy between extramurally funded research and academics. We have developed our research enterprise to the benefit of our students, allowing them to use
The SIF is funded largely by the National Institutes of Health
Consortium Principal Investigator
F. David Horgen, Ph.D. (dhorgen@hpu.edu)
Research Facilities Manager
Dr. Kosuke Ohgo. (kohgo@hpu.edu)
Contributing Faculty
Yongli Chen, Ph.D. (ychen@hpu.edu)
Gideon Berger, Ph.D. (gberger@hpu.edu)
Lei Wang, Ph.D. (lwang@hpu.edu)
RESEARCH TECHNICIAN
Ellis Akana (elakana@hpu.edu)
Research Assistants
Savana Vander Schuit
Jessie Nguyen
Kacy Kim
Olivia Honaker
AMber Aduja
Richelle Alfonso
Chloe Todd
STUDENT INSTRUMENTATION MENTORING PROGRAM
Amber Aduja (Co-Lead mentor)
Chloe Todd
Elena Polizotto
Gina Muench
Ivy Ammerman
Jessie Nguyen
Kacy Kim
Kaley Saludares
Kirah Boynton-Brown
MaxAllen Ponce
Olivia Honaker
Richelle Alfonso
Savana Vander Schuit (Co-Lead mentor)
SImone Duran-Nyers
Xiao Yi Wang-Beckvall
Hawaii Pacific University operates a wide range of scientific equipment in support of instruction and research. The various instruments are housed and maintained by the Shared Instrumentation Facility (SIF), the Instructional Science Laboratories, or by individual investigators. An important strategy in the
HPU's Research Instrumentation
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
FPLC (fast protein liquid chromatography) |
Protein purification |
MP 401 |
|
|
GC-MS (gas chromatograph-mass spectrometer) |
Autosampler; electron impact source; quadrapole analyzer; flame ionization detector |
HL 214 |
|
|
Infrared spectrometer |
Measures infrared absorption |
HL 102B |
|
|
Isocratic HPLC (high-performance liquid chromatography) |
Purifies extracts or synthetic products |
HL 214 |
|
|
LC-MS (liquid chromatography-mass spectrometer) |
Electrospray and atmospheric pressure chemical ionization, positive and negative mode; MS/MS; high-resolution MS. autosampler; fraction collector |
HL 214 |
|
|
NMR spectrometer |
1H, 13C, 15N, 31P detection with gradients, one- and two-dimensional experiments |
HL 102D |
|
|
Patch clamp electrophysiology system |
Measures flux of ions across cell membranes |
HL 102A |
|
|
Plate reader |
Fluorescence/absorbance/luminescence plate reader |
MP 402 |
|
|
Polarimeter |
Optical rotation measurements |
HL 102A |
|
|
Preparative HPLC (high-performance liquid chromatography) |
Flow up to 100 mL/min; manual injector, multi-wavelength UV detector, fraction collector |
HL 214 |
|
|
Scanning robotic plate reader |
Fluorescence/absorbance/luminescence plate reader with 8 channel fluidics |
HL 102A |
|
|
Solvent extractor |
Extraction of |
HL 214 |
|
|
Solvent purification System |
Production of anhydrous solvent |
HL 102B |
|
|
Spectrofluorometer |
Fluorescence/absorbance/luminescence plate reader |
MP 402 |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
Autoclave |
Sterilization of materials and waste |
HL 102A |
|
|
Autoclave |
Sterilization of materials and waste |
MP 402 |
|
|
Bead homogenizer |
Disruption of biological samples for extraction |
HL 102A |
|
|
Benchtop spectrometer |
Measure chemical absorption |
HL 211A |
|
|
Benchtop spectrometer |
Measures UV absorption |
MP 401 |
|
|
Centrifuge |
Centrifugation of cell culture samples |
HL 102A |
|
|
Centrifuge |
Centrifugation of reagents and samples |
MP 401 |
|
|
Centrifuge |
Centrifugation of samples |
MP 402 |
|
|
CO2 incubator |
Controls humidity, CO2 concentrations, and temperature of cell cultures |
HL 102A |
|
|
CO2 incubator |
Controls humidity, CO2 concentrations, and temperature of cell cultures |
MP 403 |
|
|
Electrophoresis system |
Separation of DNA and protein |
HL 102A |
|
|
Electrophoresis system |
Separation of DNA and protein |
MP 401 |
|
|
Orbital shaker |
Mixing chemicals and reagents |
MP 401 |
|
|
Plate washer |
96 well plate washing, aspirating and dispensing |
HL 102A |
|
|
Refrigerated centrifuge |
Centrifugation of samples (mammalian cells or non-viable samples only) |
HL 214 |
|
|
Shaking incubator |
Heating samples and reagents |
HL 214 |
|
|
Shaking incubator |
Heating samples and reagents |
MP 401 |
|
|
Thermocycler |
Incubations and polymerase chain reaction experiments |
HL 102B |
|
|
Thermocycler |
Incubations and polymerase chain reaction experiments |
HL 214 |
|
|
Thermocycler |
Incubations and polymerase chain reaction experiments |
MP 402 |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
Analytical balance |
Measures sample mass |
HL 102A |
|
|
Analytical balance |
Measures sample mass |
HL 102A |
|
|
Analytical balance |
Measures sample mass |
HL 102B |
|
|
Analytical balance |
Measures sample mass |
HL 212 |
|
|
Analytical balance |
Measures sample mass |
HL 102D |
|
|
Analytical balance |
Measures sample mass |
MP 402 |
|
|
Benchtop |
Measures UV absorption |
HL 102A |
|
|
Benchtop |
Measures UV absorption |
MP 402 |
|
|
Calorimeter |
Measures heat evolved by chemical reactions |
HL 103 |
|
|
Calorimeter |
Measures heat evolved by chemical reactions |
HL 103 |
|
|
Centrifuge |
Centrifugation of samples |
MP 401 |
|
|
Convection oven |
Oven to dry samples |
HL 102B |
|
|
Convection oven |
Oven to dry samples |
HL 212A |
|
|
Freeze-dryer |
Freeze dry various research sample and dry residual water in samples |
HL 214 |
|
|
Incubator |
Oven to dry samples |
MP 400 |
|
|
Incubator |
Incubator for bacterial cultures |
MP 401 |
|
|
Oven |
Oven to dry samples/equipment |
HL 214 |
|
|
Oven |
Oven to dry samples |
HL 214 |
|
|
Refrigerated centrifuge |
Centrifugation of samples |
HL 102A |
|
|
Rotary evaporator |
Evaporates solvents from solutions |
HL 214 |
|
|
Rotary evaporator |
Evaporates solvents from solutions |
HL 214 |
|
|
Top loading balance |
Measures sample mass |
HL 102D |
|
|
Top loading balance |
Measures sample mass |
HL 214 |
|
|
UV/VIS spectrometer |
Measure ultraviolet and visible light absorption |
HL 211A |
|
|
UV/VIS spectrometer |
Measure ultraviolet and visible light absorption |
HL 211A |
|
|
Vacuum evaporation system |
Evaporates solvents from solutions |
HL 214 |
|
|
Vacuum evaporation system |
Evaporates solvents from solutions |
HL 214 |
|
|
Vacuum evaporation system |
Evaporates solvents from solutions |
HL 214 |
|
|
Vacuum evaporation system |
Evaporates solvents from solutions |
HL 214 |
|
|
Water systems |
Produces Type II and Type I water |
HL 102A |
|
|
Water systems |
Produces Type II water |
HL 102D |
|
|
Water systems |
Produces Type II and Type I water |
MP 401 |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
Pipette puller |
Glass pipettes for electrophysiology |
HL 102A |
|
|
Pipette puller |
Glass pipettes for electrophysiology |
HL 102A |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
Freezer |
Sample, cell, and reagent storage |
HL 102A |
|
|
Freezer |
Sample, cell, and reagent storage |
HL 102A |
|
|
Freezer |
Sample, cell, and reagent storage |
HL 102D |
|
|
Freezer |
Sample, cell, and reagent storage |
HL 214 |
|
|
Freezer |
Sample, cell, and reagent storage |
HL 214 |
|
|
Freezer |
Sample, cell, and reagent storage |
HL 214 |
|
|
Freezer |
Sample, cell, and reagent storage |
MP 402 |
|
|
Freezer |
Sample, cell, and reagent storage |
MP 403 |
|
|
Refrigerator |
Sample, cell, and reagent storage |
HL 102A |
|
|
Refrigerator |
Chemical storage |
HL 102B |
|
|
Refrigerator |
Sample storage |
HL 211A |
|
|
Refrigerator |
Sample, cell, and reagent storage |
MP 401 |
|
|
Refrigerator |
Sample, cell, and reagent storage |
MP 403 |
|
|
Refrigerator |
Sample, cell, and reagent storage |
MP 403 |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
Biological safety cabinet laminar flow hood |
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) |
MP 401 |
|
|
Biological safety cabinet laminar flow hood |
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) |
MP 403 |
|
|
Biological safety cabinet laminar flow hood |
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) |
HL 102A |
|
|
Ductless fume hood |
Clean environment for preparation of PCR and seeding microbial cultures |
HL 214 |
|
|
Fume hood |
Safe removal of chemical fumes |
MP 401 |
|
|
Fume hood |
Safe removal of chemical fumes |
HL 102D |
|
|
Fume hood |
Safe removal of chemical fumes |
HL 214 |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
CTD |
Conductivity, temperature and depth for oceanographic water analyses |
HPU, R/V KAHOLO |
|
|
Current profiler |
Measures marine current |
HL 211A |
|
|
R/V Kaholo |
Teaching and research vessel |
Kaneohe Bay, Coconut Island |
|
|
Skiff, motor, trailer |
Research craft |
Kaneohe Bay, Coconut Island |
|
Instrument |
Description |
Contact |
Location |
|---|---|---|---|
|
Camera |
General use camera |
HL 213 |
|
|
Camera |
General use camera |
HL 211A |
|
|
Compound microscope |
General use camera |
HL 102A |
|
|
Compound microscope |
General use camera |
HL 213 |
|
|
Inverted microscope |
Imaging cells in culture |
HL 102A |
|
|
Inverted microscope |
Imaging cells in culture |
HL 108 |
|
|
Microscope |
General use microscope |
HL 102A |
|
|
Microscope |
General use microscope |
MP 403 |
|
|
Microscope, stereoscope |
General use dissecting microscope |
HL 211A |