Natural Sciences

Scientific Instrumentation at Hawaii Pacific University

Horgen_lab

Hawaii Pacific University operates a wide range of scientific equipment in support of instruction and research.  The various instruments are housed and maintained by the Shared Instrumentation Facility (SIF), the Instructional Science Laboratories, or by individual investigators. An important strategy in development of instrumental capabilities at HPU has been to integrate research-grade equipment into our academic curriculum, thereby exposing all undergraduate science majors to state-of-the-art high tech instruments in their laboratory course work.  Collectively, the instrumentation facilities at HPU have been supported by the National Science Foundation, the National Institutes of Health, and private funding. The table below catalogues HPU’s shared instrumentation, their location, and the person to contact for details on availability and potential training.

For more info on the Shared Instrumentation Facility click here.

HPU’s Major Research Instrumentation

Instrument Capabilities Contact Location
-80C freezer:
VWR
Sample, cell, and reagent storage Chen, Yongli HL 102A
-80C freezer:
Thermo Scientific Revco Value Plus
Sample storage West, Kristi HL 214
Analytical balance:
Sartorius, CPA124S
Measures sample mass Allen, Patricia HL 212
Analytical balance:
Sartorius, CP124S
Measures sample mass Allen, Patricia HL 212
Analytical balance:
Sartorius, CP124S
Measures sample mass Allen, Patricia HL 102B
Analytical Balance:
Sartorius, CP124S
Measures sample mass Chen, Yongli HL 102A
Analytical Balance:
Sartorius, CP225D
Measures sample mass Horgen, David HL 102D
Autoclave:
Heto top loading
Sterilization of materials and waste Chen, Yongli HL 102A
Benchtop spectrometer:
Bio-Rad Labortories, Smartspec Plus
Measure chemical absorption Allen, Patricia HL 211A
Biological Safety Cabinet Laminar flow hood:
6 ft: Labconco
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) Chen, Yongli HL 102A
Camera:
Infinity, 2-3C
General use camera Allen, Patricia HL 213
Camera:
Nikon DS-L1
Teaching labs Allen, Patricia HL 211A
Compound Microscope:
Nikon E200
Multiple uses, general use microscope Chen, Yongli HL 102A
Convection oven:
Blue-M, MO1450SA
Oven to dry samples Allen, Patricia HL 102B
Convection oven:
Lindberg/BlueM, MO1450A-1
Oven to dry samples Allen, Patricia HL 212A
CTD:
Sea-Bird Electronics, SBE-19-01
Conductivity, temperature and depth for oceanographic water analyses Allen, Patricia HPU, R/V KAHOLO
Current Profiler:
Nortek USA, Aquadop 1 MHz
Measures marine current Allen, Patricia HL 211A
Fourier Transform-Infrared Spectrophotometer: 
Perkin-Elmer Spectrum RX1
Measure infrared absorption of solutions, solids, and dry films Allen, Patricia HL 102B
Freeze-dryer: 
Heto L3300; 6-L capacity
Freeze dry various research sample and dry residual water in samples Horgen, David HL 214
GC-MS (gas chromatograph-mass spectrometer):
Agilent 7890A GC; 5975C MS
Autosampler; electron impact source; quadrapole analyzer; flame ionization detector Fang, Jiasong HL 214
Inverted Microscope:
Nikon TS100
Imaging cells in culture Chen, Yongli HL 102A
Irradiameter:
Kahl Scientific, 268WD305
In situ measurement of light attenuation Allen, Patricia HL 211A
Isocratic HPLC: 
Shimadzu LC-6A and D-Star LC; analytical and semipreparative capable; chart integrator, single wavelength UV detector
Purifies extracts or synthetic products Horgen, David HL 214
LC-MS (liquid chromatography-mass spectrometer) with photodiode array (PDA) and evaporative light scattering (ELSD) detectors:
Agilent 1100 LC; Thermo-Finnigan LCQ Deca XP Max MS, Surveyor PDA; Sedere Sedex 75 ELSD
Electrospray and atmospheric pressure chemical ionization, positive and negative mode; MS/MS up to MS10. ELSD and PDA detection; autosampler; fraction collector Horgen, David HL 102D
Microscope, compound:
Olympus, CX31
General use microscope Allen, Patricia HL 213
Microscope, Stereoscope:
Nikon SMZ-800
General use dissecting microscope Allen, Patricia HL 211A
Multi-corer, mini:
Ocean Instruments/2002, MC-200-4
Marine sediment corer Allen, Patricia HPU, R/V KAHOLO, or wet lab 
NMR spectrometer:
300 MHz Varian
1H, 13C, 15N, 31P detection with gradients, one- and two-dimensional experiments Horgen, David HL 102D
Patch clamp electrophysiology system Measures flux of ions across cell membranes Chen, Yongli HL 102A
PCR (polymerase chain reaction instrument) Amplifies DNA Chen, Yongli HL 102A
PCR Thermocycler:
Hybaid, OMN-E Thermal cycler
For PCR (Polymerase chain reaction) Allen, Patricia HL 102B
Plate Washer: 
BioTek ELx-50
96 well plate washing, aspirating and dispensing Chen, Yongli HL 102A
Polarimeter:
Rudolph Research Analytical Autopol III
optical rotation measurements Horgen, David HL 102A
Preparative HPLC:
Agilent 1100 preparative system
Flow up to 100 mL/min; manual injector, multi-wavelength UV detector, fraction collector Horgen, David HL 214
R/V KAHOLO:
Murakami Power Catamaran, 42'
Teaching and research vessel Allen, Patricia HL/ Coconut Island
Refrigerated centrifuge: 
Beckman and Sigma
Centrifugation of samples (mammalian cells or non-viable samples only) Chen, Yongli HL 102A
Refrigerated centrifuge: 
Eppendorf Centrifuge 5810 R 15 amp version
Centrifugation of samples (mammalian cells or non-viable samples only) Chen, Yongli HL 102A
Refrigerated shaking incubator Controls temperature and provides shaking for microbial cultures Chen, Yongli HL 102A
Refrigerator, 2-door:
Howard-McCray, HC45
Sample storage Allen, Patricia HL 211A
Refrigerator, chemical storage:
VWR International, R421FA14
Chemical storage Allen, Patricia HL 102B
Rotary Evaporator: 
IKA RV 10 CV S1
Evaporates solvents from solutions Berger, Gideon HL 214
Rotary Evaporator: 
Heidolph LR4002
Evaporates solvents from solutions Berger, Gideon HL 214
Scanning robotic plate reader:
Molecular Devices FlexStation III
Fluorescence/absorbance/luminescence plate reader with 8 channel fluidics Chen, Yongli HL 102A
Scout II Data System:
Hydrolab Corp, Scout 2
Water quality analyses (pH/O2/conductivity/temperature/depth) Allen, Patricia HL 211A
Skiff, motor, trailer:
Livingston 12' skiff, 8 hp motor
Research craft Allen, Patricia HL, Coconut Island
Solvent purification system:
LC Technology Solutions SPBT-101
Dries and purifies tetrahydrofuran Berger, Gideon HL 102D
Thermocycler: 
Biorad MJ Mini; 48-welL
Performs polymerase chain reaction experiments Chen, Yongli HL 102A
Top Loading Balance: 
Denver Instrument SI-403
Measures sample mass Horgen, David HL 214
Top Loading Balance: 
Sartorius CP2202S
Measures sample mass Horgen, David HL 102D
Two CO2 water jacketed incubators:
Shelton
Controls humidity, CO2 concentrations, and temperature of cell cultures Chen, Yongli HL 102A
Ultraviolet-Visible Spectrophotometer: 
Perkin-Elmer EZ210; dual beam spectrometer
Measures UV and visible light absorption of  liquids and solutions Allen, Patricia HL  211
UV/VIS Spectrometer:
Perkin Elmer Lambda 25
Measure ultraviolet and visible light absorption Allen, Patricia HL 211A
UV/VIS Spectrometer: 
Perkin Elmer Lambda 20
Measure ultraviolet and visible light absorption Allen, Patricia HL 211A
Vacuum Evaporation System: 
Savant SpeedVac concentrator; -80C cold trap
Evaporates solvents from solutions Horgen, David HL 214
Van Veen grab: 
Wildlife Supply Co. 1775-A10
Marine sediment corer Allen, Patricia HPU, R/V KAHOLO
Water systems:
Millipore Elix and MilliQ
Produces Type I water Chen, Yongli HLs 102A
Water systems:
Millipore Elix and MilliQ
Produces Type I water Horgen, David HLs 102D