Scientific Instrumentation at Hawaii Pacific University
Hawaii Pacific University operates a wide range of scientific equipment in support of instruction and research. The various instruments are housed and maintained by the Shared Instrumentation Facility (SIF), the Instructional Science Laboratories, or by individual investigators. An important strategy in development of instrumental capabilities at HPU has been to integrate research-grade equipment into our academic curriculum, thereby exposing all undergraduate science majors to state-of-the-art high tech instruments in their laboratory course work. Collectively, the instrumentation facilities at HPU have been supported by the National Science Foundation, the National Institutes of Health, and private funding. The Table below catalogues HPU’s shared instrumentation, their location, and the person to contact for details on availability and potential training.
For more info on the Shared Instrumentation Facility click here.
HPU’s Major Research Instrumentation
|
Instrument |
Capabilities |
Person in Charge |
Location |
|
NMR spectrometer: 300 MHz Varian |
1H, 13C, 15N, 31P detection with gradients, one- and two-dimensional experiments |
HL 102D |
|
|
Scanning robotic plate reader: Molecular Devices FlexStation III |
Fluorescence/absorbance/luminescence plate reader with 8 channel fluidics |
HL 102A |
|
|
LC-MS (liquid chromatography-mass spectrometer) with photodiode array (PDA) and evaporative light scattering (ELSD) detectors: Agilent 1100 LC; Thermo-Finnigan LCQ Deca XP Max MS, Surveyor PDA; Sedere Sedex 75 ELSD |
Electrospray and atmospheric pressure chemical ionization, positive and negative mode; MS/MS up to MS10. ELSD and PDA detection; autosampler; fraction collector |
HL 102D |
|
|
Preparative HPLC: Agilent 1100 preparative system |
Flow up to 100 mL/min; manual injector, multi-wavelength UV detector, fraction collector |
HL 214 |
|
|
GC-MS (gas chromatograph-mass spectrometer): Agilent 7890A GC; 5975C MS |
Autosampler; electron impact source; quadrapole analyzer; flame ionization detector |
HL 214 |
|
|
Polarimeter: Rudolph Research Analytical Autopol III |
optical rotation measurements |
HL 102A |
|
|
Solvent purification system: LC Technology Solutions SPBT-101 |
Dries and purifies tetrahydrofuran |
Gideon Berger |
HL 102D |
|
Ultraviolet-Visible Spectrophotometer: Perkin-Elmer EZ210; dual beam spectrometer |
Measures UV and visible light absorption of liquids and solutions |
HL 211 |
|
|
Freeze-dryer: Heto L3300; 6-L capacity |
Freeze dry various research sample and dry residual water in samples |
HL 214 |
|
|
Vacuum Evaporation System: Savant SpeedVac concentrator; -80C cold trap |
Evaporates solvents from solutions |
HL 214 |
|
|
Isocratic HPLC: Shimadzu LC-6A and D-Star LC; analytical and semipreparative capable; chart integrator, single wavelength UV detector |
Purifies extracts or synthetic products |
HL 214 |
|
|
Fourier Transform-Infrared Spectrophotometer: Perkin-Elmer Spectrum RX1, |
Measure infrared absorption of solutions, solids, and dry films |
HL 102B |
|
|
Rotary Evaporator: IKA RV 10 CV S1 |
Evaporates solvents from solutions |
Gideon Berger |
HL 214 |
|
Rotary Evaporator: Heidolph LR4002 |
Evaporates solvents from solutions |
Gideon Berger |
HL 214 |
|
Patch clamp electrophysiology system: |
Measures flux of ions across cell membranes |
HL 102A |
|
|
Thermocycler: Biorad MJ Mini; 48-welL |
Performs polymerase chain reaction experiments |
HL 102A |
|
|
PCR (polymerase chain reaction instrument) |
Amplifies DNA |
HL 102A |
|
| Two CO2 water jacketed incubators: Shelton |
Controls humidity, CO2 concentrations, and temperature of cell cultures |
HL 102A |
|
|
Biological Safety Cabinet Laminar flow hood: 6 ft: Labconco, |
Provide aseptic environment for manipulating cells in cultures (for mammalian cells only) |
HL 102A |
|
|
Refrigerated centrifuge: Beckman and Sigma |
Centrifugation of samples (mammalian cells or non-viable samples only) |
HL 102A |
|
|
Refrigerated centrifuge: Eppendorf Centrifuge 5810 R 15 amp version |
Centrifugation of samples (mammalian cells or non-viable samples only) |
HL 102A |
|
|
Refrigerated shaking incubator: |
Controls temperature and provides shaking for microbial cultures |
HL 102A |
|
|
Water systems: Millipore Elix and MilliQ |
Produces Type I water |
HLs 102A |
|
|
Water systems: Millipore Elix and MilliQ |
Produces Type I water |
HLs 102D |
|
|
Autoclave: Heto top loading |
Sterilization of materials and waste |
HL 102A |
|
|
Inverted Microscope: Nikon TS100 |
Imaging cells in culture |
HL 102A |
|
|
Compound Microscope: Nikon E200 |
Multiple uses, general use microscope |
HL 102A |
|
|
Plate Washer: BioTek ELx-50 |
96 well plate washing, aspirating and dispensing |
HL 102A |
|
|
-80C freezer: VWR |
Sample, cell, and reagent storage |
HL 102A |
|
|
-80C freezer: Thermo Scientific Revco Value Plus |
Sample storage |
HL 214 |
|
|
Top Loading Balance: Denver Instrument SI-403 |
Measures sample mass |
HL 214 |
|
|
Top Loading Balanc: Sartorius CP2202S |
Measures sample mass |
HL 102D |
|
|
Analytical Balance: Sartorious CP225D |
Measures sample mass |
HL 102D |
|
|
Analytical Balance: Sartorious CP124S |
Measures sample mass |
HL 102A |
|
|
UV/VIS Spectrometer: Perkin Elmer Lambda 20 |
Measure ultraviolet and visible light absorption |
HL 211A |
|
|
UV/VIS Spectrometer: Perkin Elmer Lambda 25 |
Measure ultraviolet and visible light absorption |
HL 211A |
|
|
Van Veen grab: Wildlife Supply Co. 1775-A10 |
Marine sediment corer |
HPU R/V KAHOLO
|
|
|
CTD: Sea-Bird Electronics SBE-19-01 |
Conductivity, temperature and depth for oceanographic water analyses |
HPU R/V KAHOLO
|
|
|
Analytical balance: Sartorius CPA124S |
Measures sample mass |
HL 212 |
|
|
Analytical balance: Sartorius CP124S |
Measures sample mass |
HL 212 |
|
|
Analytical balance: Sartorius CP124S |
Measures sample mass |
HL 102B |
|
|
Current Profiler: Nortek USA Aquadop 1 MHz |
Measures marine current |
HL 211A |
|
|
Irradiameter: Kahl Scientific 268WD305 |
In situ measurement of light attenuation |
HL 211A |
|
|
Multi-corer, mini: Ocean Instruments/2002 MC-200-4 |
Marine sediment corer |
HPU R/V KAHOLO Or wet lab
|
|
|
Convection oven: Blue-M MO1450SA |
Oven to dry samples |
HL 102B |
|
|
Convection oven: Lindberg/BlueM MO1450A-1 |
Oven to dry samples |
HL 212A |
|
|
Benchtop spectrometer: Bio-Rad Labortories Smartspec Plus |
Measure chemical absorption |
HL 211A |
|
|
PCR Thermocycler: Hybaid OMN-E Thermal cycler |
For PCR (Polymerase chain reaction) |
HL 102B |
|
|
Refrigerator, chemical storage: VWR International R421FA14 |
Chemical storage |
HL 102B |
|
|
Refrigerator, 2-door: Howard-McCray HC45 |
Sample storage |
HL 211A |
|
|
Scout II Data System: Hydrolab Corp Scout 2 |
Water quality analyses (pH/O2/conductivity/temperature/depth) |
HL 211A |
|
|
Microscope, compound : Olympus CX31 |
General use microscope |
HL 213 |
|
|
Camera: Infinity 2-3C |
General use camera |
HL 213 |
|
|
Microscope, Stereoscope: Nikon SMZ-800 |
General use dissecting microscope |
HL 211A |
|
|
Camera: Nikon DS-L1 |
Teaching labs |
HL 211A |
|
|
R/V KAHOLO: Murakami Power Catamaran, 42' |
Teaching and research vessel |
HL/ Coconut Island
|
|
|
Skiff, motor, trailer: Livingston 12' skiff, 8 hp motor |
Research craft |
HL/ Coconut Island
|
