Natural Sciences

Scientific Instrumentation at Hawaii Pacific University

Horgen_lab

Hawaii Pacific University operates a wide range of scientific equipment in support of instruction and research.  The various instruments are housed and maintained by the Shared Instrumentation Facility (SIF), the Instructional Science Laboratories, or by individual investigators. An important strategy in development of instrumental capabilities at HPU has been to integrate research-grade equipment into our academic curriculum, thereby exposing all undergraduate science majors to state-of-the-art high tech instruments in their laboratory course work.  Collectively, the instrumentation facilities at HPU have been supported by the National Science Foundation, the National Institutes of Health, and private funding. The Table below catalogues HPU’s shared instrumentation, their location, and the person to contact for details on availability and potential training.

For more info on the Shared Instrumentation Facility click here.

HPU’s Major Research Instrumentation

Instrument

Capabilities

Person in Charge

Location

NMR spectrometer:

300 MHz Varian

1H, 13C, 15N, 31P detection with gradients, one- and two-dimensional experiments

David Horgen

HL 102D

Scanning robotic plate reader:

Molecular Devices FlexStation III

Fluorescence/absorbance/luminescence plate reader with 8 channel fluidics

Yongli Chen

HL 102A

LC-MS (liquid chromatography-mass

spectrometer) with photodiode array

(PDA) and evaporative light scattering (ELSD) detectors:

Agilent 1100 LC; Thermo-Finnigan LCQ Deca XP Max MS, Surveyor PDA; Sedere Sedex 75 ELSD

Electrospray and atmospheric pressure chemical ionization, positive and negative mode; MS/MS up to MS10. ELSD and PDA detection; autosampler; fraction collector

David Horgen

HL 102D

Preparative HPLC:

Agilent 1100 preparative system

Flow up to 100 mL/min; manual injector, multi-wavelength UV detector, fraction collector

Justin Reinicke

HL 214

GC-MS (gas chromatograph-mass

spectrometer):

Agilent 7890A GC; 5975C MS

Autosampler; electron impact source; quadrapole analyzer; flame ionization detector

Jiasong Fang

HL 214

Polarimeter:

Rudolph Research Analytical Autopol III

optical rotation measurements

Justin Reinicke

HL 102A

Solvent purification system:

LC Technology Solutions SPBT-101

Dries and purifies tetrahydrofuran

Gideon Berger   

HL 102D

Ultraviolet-Visible Spectrophotometer: Perkin-Elmer EZ210; dual beam spectrometer

Measures UV and visible light absorption of  liquids and solutions

Patricia Allen

HL  211

Freeze-dryer: Heto L3300; 6-L capacity

Freeze dry various research sample and dry residual water in samples

Justin Reinicke

HL 214

Vacuum Evaporation System: Savant SpeedVac concentrator; -80C cold trap

Evaporates solvents from solutions

Justin Reinicke

HL 214

Isocratic HPLC: Shimadzu LC-6A and D-Star LC; analytical and semipreparative capable; chart integrator, single wavelength UV detector

Purifies extracts or synthetic products

Justin Reinicke

HL 214

Fourier Transform-Infrared Spectrophotometer: Perkin-Elmer Spectrum RX1,

Measure infrared absorption of solutions, solids, and dry films

Patricia Allen

HL 102B

Rotary Evaporator: IKA RV 10 CV S1

Evaporates solvents from solutions

Gideon Berger   

HL 214

Rotary Evaporator: Heidolph LR4002

Evaporates solvents from solutions

Gideon Berger   

HL 214

Patch clamp electrophysiology system:

Measures flux of ions across cell membranes

Yongli Chen

HL 102A

Thermocycler: Biorad MJ Mini; 48-welL

Performs polymerase chain reaction experiments

Yongli Chen

HL 102A

PCR (polymerase chain reaction instrument)

Amplifies DNA

Yongli Chen

HL 102A

Two CO2 water jacketed incubators: Shelton

Controls humidity, CO2 concentrations, and temperature of cell cultures

Yongli Chen

HL 102A

Biological Safety Cabinet

Laminar flow hood: 6 ft: Labconco,

Provide aseptic environment for manipulating cells in cultures (for mammalian cells only)

Yongli Chen

HL 102A

Refrigerated centrifuge: Beckman and Sigma

Centrifugation of samples (mammalian cells or non-viable samples only)

Yongli Chen

HL 102A

Refrigerated centrifuge: Eppendorf Centrifuge 5810 R 15 amp version

Centrifugation of samples (mammalian cells or non-viable samples only)

Yongli Chen

HL 102A

Refrigerated shaking incubator:

Controls temperature and provides shaking for microbial cultures

Yongli Chen

HL 102A

Water systems:

Millipore Elix and MilliQ

Produces Type I water

Yongli Chen 

HLs 102A

Water systems:

Millipore Elix and MilliQ

Produces Type I water

Justin Reinicke

HLs 102D

Autoclave:

Heto top loading

Sterilization of materials and waste

Yongli Chen

HL 102A

Inverted Microscope:

Nikon TS100

Imaging cells in culture

Yongli Chen

HL 102A

Compound Microscope:

Nikon E200

Multiple uses, general use microscope

Yongli Chen 

HL 102A

Plate Washer: BioTek ELx-50

96 well plate washing, aspirating and dispensing

Yongli Chen

HL 102A

-80C freezer:

VWR

Sample, cell, and reagent storage

Yongli Chen

HL 102A

-80C freezer:

Thermo Scientific Revco Value Plus

Sample storage

Kristi West

HL 214

Top Loading Balance: Denver Instrument SI-403

Measures sample mass

Justin Reinicke

HL 214

Top Loading Balanc: Sartorius

CP2202S

Measures sample mass

Justin Reinicke

HL 102D

Analytical Balance: Sartorious

CP225D

Measures sample mass

Justin Reinicke

HL 102D

Analytical Balance: Sartorious

CP124S

Measures sample mass

Yongli Chen

HL 102A

UV/VIS Spectrometer: Perkin Elmer

Lambda 20

Measure ultraviolet and visible light absorption

Patricia Allen

HL 211A

UV/VIS Spectrometer: Perkin Elmer

Lambda 25

Measure ultraviolet and visible light absorption

Patricia Allen

HL 211A

Van Veen grab: Wildlife Supply Co. 1775-A10

Marine sediment corer

Patricia Allen

HPU

R/V KAHOLO

CTD:

Sea-Bird Electronics

SBE-19-01

Conductivity, temperature and depth for oceanographic water analyses

Patricia Allen

HPU

R/V KAHOLO

 

Analytical balance:

Sartorius

CPA124S

Measures sample mass

Patricia Allen

HL 212

Analytical balance:

Sartorius

CP124S

Measures sample mass

Patricia Allen

HL 212

Analytical balance:

Sartorius

CP124S

Measures sample mass

Patricia Allen

HL 102B

Current Profiler:

Nortek USA

Aquadop 1 MHz

Measures marine current

Patricia Allen

HL 211A

Irradiameter:

Kahl Scientific

268WD305

In situ measurement of light attenuation

Patricia Allen

HL 211A

Multi-corer, mini:

Ocean Instruments/2002

MC-200-4

Marine sediment corer

Patricia Allen

HPU

R/V KAHOLO

Or wet lab

Convection oven:

Blue-M

MO1450SA

Oven to dry samples

Patricia Allen

HL 102B

Convection oven:

Lindberg/BlueM

MO1450A-1

Oven to dry samples

Patricia Allen

HL 212A

Benchtop spectrometer:

Bio-Rad Labortories

Smartspec Plus

Measure chemical absorption

Patricia Allen

HL 211A

PCR Thermocycler:

Hybaid

OMN-E Thermal cycler

For PCR (Polymerase chain reaction)

Patricia Allen

HL 102B

Refrigerator, chemical storage:

VWR International

R421FA14

Chemical storage

Patricia Allen

HL 102B

Refrigerator, 2-door:

Howard-McCray

HC45

Sample storage

Patricia Allen

HL 211A

Scout II Data System:

Hydrolab Corp

Scout 2

Water quality analyses (pH/O2/conductivity/temperature/depth)

Patricia Allen

HL 211A

Microscope, compound :

Olympus

CX31

General use microscope

Patricia Allen

HL 213

Camera:

Infinity

2-3C

General use camera

Patricia Allen

HL 213

Microscope, Stereoscope:

Nikon

SMZ-800

General use dissecting microscope

Patricia Allen

HL 211A

Camera:

Nikon

DS-L1

Teaching labs

Patricia Allen

HL 211A

R/V KAHOLO:

Murakami

Power Catamaran, 42'

Teaching and research vessel

Patricia Allen

HL/ Coconut Island

 

Skiff, motor, trailer:

Livingston

12' skiff, 8 hp motor

Research craft

Patricia Allen

HL/ Coconut Island